MATRIX ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY
\mˈe͡ɪtɹɪks ɐsˈɪstɪd lˈe͡ɪzə dɪsˈɔːpʃən ˌa͡ɪəna͡ɪzˈe͡ɪʃən mˈas spɛktɹˈɒmətɹi], \mˈeɪtɹɪks ɐsˈɪstɪd lˈeɪzə dɪsˈɔːpʃən ˌaɪənaɪzˈeɪʃən mˈas spɛktɹˈɒmətɹi], \m_ˈeɪ_t_ɹ_ɪ_k_s ɐ_s_ˈɪ_s_t_ɪ_d l_ˈeɪ_z_ə d_ɪ_s_ˈɔː_p_ʃ_ə_n ˌaɪ_ə_n_aɪ_z_ˈeɪ_ʃ_ə_n m_ˈa_s s_p_ɛ_k_t_ɹ_ˈɒ_m_ə_t_ɹ_i]\
Definitions of MATRIX ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY
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A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
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